Affiliation:
1. Department of Cancer, Immunology and AIDS, Dana-Farber Cancer Institute
2. Department of Medicine, Harvard Medical School, Boston, Massachusetts
3. Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska
4. Department of Pediatrics, University Teaching Hospital, Lusaka, Zambia
Abstract
ABSTRACT
Although human immunodeficiency virus type 1 (HIV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir.
15:
1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol.
75:
4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures;
gag
,
pol
, and
env
consistently mapped within clade C. Interestingly,
gag
resembled Asian isolates, whereas
pol
and
env
resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology