Affiliation:
1. Institute of Molecular Biology and Genetics, Mahidol University, Thailand
Abstract
ABSTRACT
Dengue virus, the causative agent of dengue fever, dengue shock syndrome, and dengue hemorrhagic fever, infects susceptible cells by initially binding to a receptor(s) located on the host cell surface. Evidence to date suggests that receptor usage may be cell and serotype specific, and this study sought to identify dengue virus serotype 1 binding proteins on the surface of liver cells, a known target organ. By using a virus overlay protein binding assay (VOPBA), in both nondenaturing and denaturing gel systems, a putative dengue virus serotype 1 binding protein of approximately 37 kDa expressed on the surface of liver (HepG2) cells was identified. Mass spectrometry analysis identified a candidate protein, the 37/67-kDa high-affinity laminin receptor. Entry of the dengue virus serotype 1 was significantly inhibited in a dose-dependent manner by both antibodies directed against the 37/67-kDa high-affinity laminin receptor and soluble laminin. No inhibition of virus entry was seen with dengue virus serotypes 2, 3, or 4, demonstrating that the 37/67-kDa high-affinity laminin receptor is a serotype-specific receptor for dengue virus entry into liver cells.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference49 articles.
1. Ardini, E., G. Pesole, E. Tagliabue, A. Magnifico, V. Castronovo, M. E. Sobel, M. I. Colnaghi, and S. Ménard. 1998. The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution. Mol. Biol. Evol.15:1017-1025.
2. Angsubhakorn, S., S. Yoksan, A. Pradermwong, N. Nitatpattana, S. Sahaphong, and N. Bhamarapravati. 1994. Dengue-3 (16562) PGMK 33 vaccine: neurovirulence, viremia and immune responses in Macaca fascicularis. Southeast Asian J. Trop. Med. Public Health25:554-559.
3. Bielefeldt-Ohmann, H. 1998. Analysis of antibody-independent binding of dengue viruses and dengue envelop protein to human myelomonocytic cells and B-lymphocytes. Virus Res.57:63-79.
4. Bielefeldt-Ohmann, H., M. Meyer, D. R. Fitzpatrick, and J. S. Mackenzie. 2001. Dengue virus binding to human leukocyte cell lines: receptor usage differs between cell types and virus strains. Virus Res.73:81-89.
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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