A Lentiviral cDNA Library Employing Lambda Recombination Used To Clone an Inhibitor of Human Immunodeficiency Virus Type 1-Induced Cell Death-Reference-Cited by-同舟云学术

A Lentiviral cDNA Library Employing Lambda Recombination Used To Clone an Inhibitor of Human Immunodeficiency Virus Type 1-Induced Cell Death

Published:2004-10-15 Issue:20 Volume:78 Page:11352-11359
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kawano Yuji¹,Yoshida Takeshi¹²,Hieda Kuniko¹²,Aoki Jun¹²,Miyoshi Hiroyuki³,Koyanagi Yoshio²

Affiliation:

1. Department of Virology, Tohoku University Graduate School of Medicine, Sendai

2. Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto

3. BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Japan

Abstract

ABSTRACT Expression cloning technology of cDNAs is a suitable tool for identifying novel functional properties of genes. Here, we generated a lentiviral cDNA library-expressing system for human T cells based on a site-specific recombination system of phage lambda for transferring cDNA libraries with a minimum loss of its complexity. The library-transduced CD4 + T cells were challenged with wild-type human immunodeficiency virus type 1 (HIV-1), and the cells that acquired resistance to HIV-1-induced cytopathic effect (CPE) were selected. From these cells, CD14 was isolated and proved to inhibit the entry of HIV-1 and the HIV-1-induced CPE. This cloning system allows rapid identification of genes encoding novel properties in human T cells and probably other mammalian cells.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.78.20.11352-11359.2004

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