Affiliation:
1. Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121
2. Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195
3. The Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103
Abstract
ABSTRACT
RNA levels of flagellar genes in eight different genetic backgrounds were compared to that of the wild type by DNA microarray analysis. Cluster analysis identified new, potential flagellar genes, three putative methyl-accepting chemotaxis proteins, STM3138 (McpA), STM3152 (McpB), and STM3216(McpC), and a CheV homolog, STM2314, in
Salmonella
, that are not found in
Escherichia coli
. Isolation and characterization of Mu
d-lac
insertions in
cheV
,
mcpB
,
mcpC
, and the previously uncharacterized
aer
locus of
S. enterica
serovar Typhimurium revealed them to be controlled by σ
28
-dependent flagellar class 3 promoters. In addition, the
srfABC
operon previously isolated as an SsrB-regulated operon clustered with the flagellar class 2 operon and was determined to be under FlhDC control. The previously unclassified
fliB
gene, encoding flagellin methylase, clustered as a class 2 gene, which was verified using reporter fusions, and the
fliB
transcriptional start site was identified by primer extension analysis. RNA levels of all flagellar genes were elevated in
flgM
or
fliT
null strains. RNA levels of class 3 flagellar genes were elevated in a
fliS
null strain, while deletion of the
fliY
,
fliZ
, or
flk
gene did not affect flagellar RNA levels relative to those of the wild type. The
cafA
(RNase G) and
yhjH
genes clustered with flagellar class 3 transcribed genes. Null alleles in
cheV
,
mcpA
,
mcpB
,
mcpC
, and
srfB
did not affect motility, while deletion of
yhjH
did result in reduced motility compared to that of the wild type.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
131 articles.
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