Cyclo(Phe-Pro) Modulates the Expression of ompU in Vibrio spp-Reference-Cited by-同舟云学术

Cyclo(Phe-Pro) Modulates the Expression of ompU in Vibrio spp

Published:2006-03-15 Issue:6 Volume:188 Page:2214-2221
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Park Dae-Kyun¹²,Lee Ko-Eun¹²,Baek Chang-Ho¹²,Kim In Hwang¹²,Kwon Ji-Hyoun³²,Lee Won Koo³²,Lee Kyu-Ho⁴,Kim Byung-Soo⁵,Choi Sang-Ho⁶,Kim Kun-Soo¹²

Affiliation:

1. Departments of Life Science

2. Interdisciplinary Program of Integrated Biotechnology, Sogang University, Sinsoo-Dong 1, Mapo-Gu, Seoul 121-742, South Korea

3. Chemistry

4. Department of Environmental Science, Hankuk University of Foreign Studies, Yongin 449-791, South Korea

5. Department of Applied Statistics, Yonsei University, Seoul 120-749, South Korea

6. School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, South Korea

Abstract

ABSTRACT Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1 H nuclear magnetic resonance analyses indicated that the compound was cyclo( l -Phe- l -Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR -null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae , V. parahaemolyticus , and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.188.6.2214-2221.2006

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