Affiliation:
1. Department of Bacteriology and Immunology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514
Abstract
A restriction-like enzyme has been purified from
Haemophilus aegyptius.
This nuclease, endonuclease Z, produces a rapid decrease in the viscosity of native calf thymus and
H. influenzae
deoxyribonucleic acids (DNA), but does not degrade homologous DNA. The specificity of endonuclease Z is different from that of the similar endonuclease isolated from
H. influenzae
(endonuclease R). The purified enzyme cleaves the double-stranded replicative form DNA of bacteriophage φX174 (φX174 RF DNA) into at least 11 specific limit fragments whose molecular sizes have been estimated by gel electrophoresis. The position of these fragments with respect to the genetic map of φX174 can be determined by using the genetic assay for small fragments of φX174 DNA.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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