Affiliation:
1. Department of Biochemistry, McGill University, Montreal 110, Quebec, Canada
Abstract
The deoxyribonucleic acid (DNA) of bacteriophage S13 was shown to be single-stranded by the criteria of reactivity with formaldehyde, dependence of optical density on ionic strength, broad temperature-absorbance profile, and lack of molar equivalence of the purine and pyrimidine bases. The DNA has a molecular weight of 1.8 × 10
6
daltons, an
S
°
20
of 24.6 in SSC (0.15
m
NaCl plus 0.015
m
sodium citrate), and a buoyant density of 1.726 g/cc in CsCl. Electron microscopy showed the molecule to be circular. S13 replicative-form DNA was shown to be a double-stranded, circular molecule with a molecular weight of 3.5 × 10
6
daltons, an
S
[ill]
of 20.7 in SSC, and a buoyant density in CsCl of 1.710 g/cc. The finding that S13 DNA is slightly more pyrimidine-rich than φX174 DNA but is indistinguishable by all other parameters supports the close genetic relationship between the two bacteriophages.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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