Affiliation:
1. Department of Microbiology, Louisiana State University, Baton Rouge, Louisiana 70803
Abstract
Steady-state continuous culture was used to optimize lean chemically defined media for a
Cellulomonas
sp. and
Bacillus cereus
strain T. Both organisms were extremely sensitive to variations in trace-metal concentrations. However, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumentation. The optimized media supported critical dilution rates of 0.571 and 0.467 h
−1
for
Cellulomonas
and
Bacillus
, respectively. These values approximated maximum growth rate values observed in batch culture.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
19 articles.
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