Protein Patterns of Growing and Starved Cells of a Marine Vibrio sp

Author:

Amy Penny S.1,Morita Richard Y.1

Affiliation:

1. Department of Microbiology and School of Oceanography, Oregon State University, Corvallis, Oregon 97331

Abstract

Fingerprint protein patterns were produced by two-dimensional polyacrylamide electrophoresis on lysed cells of a Vibrio sp., Ant-300, which were prepared from growing and starved cultures. The cells were labeled with [ 35 S]methionine during growth and subsequently starved for up to 30 days. Samples were taken at selected time points representing stages in the starvation-survival process. Unlabeled starved cells were allowed to recover in the presence of [ 35 S]methionine to determine protein changes associated with the recovery from starvation. All growth conditions produced similar protein fingerprints; however, some protein spots disappeared, whereas others were seen only during starvation. Images

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

1. Starvation-survival patterns of sixteen isolated open ocean bacteria;Amy P. S.;Appl. Environ. Microbiol.,1983

2. Starvation-survival processes of a marine vibrio;Amy P. S.;Appl. Environ. Microbiol.,1983

3. Recovery from nutrient starvation by a marine Vibrio sp;Amy P. S.;Appl. Environ. Microbiol.,1983

4. Baross J. A. J. Hanus and R. Y. Morita. 1974. Effects of hydrostatic pressure on uracil uptake ribonucleic acid synthesis and growth of three obligately psychrophilic marine vibrios Vibrio alginolyticus and Escherichia coli p. 180-202. In R. R. Colwell and R. Y. Morita (ed.) Effect of the ocean environment on microbial activities. University Park Press Baltimore.

5. Capture of arginine at low concentrations by a marine psychrophilic bacterium;Geesey G. G.;AppI. Environ. Microbiol.,1979

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