Affiliation:
1. Department of Microbiology and Medical Technology and Laboratory of Organic Geochemistry, Department of Geosciences, The University of Arizona, Tucson, Arizona 85721
Abstract
A mesophilic yeast,
Candida utilis
, and a psychrophilic yeast,
Leucosporidium stokesii
, were subjected to freeze-thaw cycling over the range 25 to -60 C. Viability after freeze-thaw stress was directly correlated with the rate of cooling and the physiological age of the cultures. Rates of glucose fermentation and oxidation could be directly correlated with viability. The optimal cooling rate for both yeast strains was 4.5 to 6.5 C/min; however, their levels of survival obtained at this optimal cooling rate varied considerably. In addition, the psychrophile was less resistant to freeze-thaw stress than was the mesophile.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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