The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus In Vivo-Reference-Cited by-同舟云学术

The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus In Vivo

Published:2019-06-25 Issue:3 Volume:10 Page:
ISSN:2161-2129
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Jackson Joseph W.¹,Hancock Trevor J.¹,LaPrade Ellen¹,Dogra Pranay¹²^ORCID,Gann Eric R.¹,Masi Thomas J.¹³,Panchanathan Ravichandran⁴,Miller William E.⁴^ORCID,Wilhelm Steven W.¹^ORCID,Sparer Tim E.¹^ORCID

Affiliation:

1. Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA

2. Columbia Center for Translational Immunology, Columbia University, New York, New York, USA

3. University of Tennessee Graduate School of Medicine, Department of Surgery, University of Tennessee Medical Center, Knoxville, Tennessee, USA

4. Department of Molecular Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA

Abstract

An adequate in vivo analysis of HCMV’s viral chemokine vCXCL-1 has been lacking. Here we generate recombinant MCMVs expressing vCXCL-1 to study vCXCL-1 function in vivo using MCMV as a surrogate. We demonstrate that vCXCL-1 increases MCMV dissemination kinetics for both primary and secondary dissemination. Additionally, we provide evidence, that the murine neutrophil is largely a bystander in the mouse’s response to vCXCL-1. We confirm the hypothesis that vCXCL-1 is a HCMV virulence factor. Infection of severely immunocompromised mice with MCMVs expressing vCXCL-1 was lethal in more than 50% of infected animals, while all animals infected with parental virus survived during a 12-day period. This work provides needed insights into vCXCL-1 function in vivo .

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.01289-19

Reference65 articles.

1. Fields Virology, 6th Edition

2. Britt W. 2007. Virus entry into host, establishment of infection, spread in host, mechanisms of tissue damage. In Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K (ed), Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, United Kingdom. https://www.ncbi.nlm.nih.gov/books/NBK47407/.

3. Congenital cytomegalovirus infection;Demmler GJ;Semin Pediatr Neurol,1994

4. The “Silent” Global Burden of Congenital Cytomegalovirus

5. Cytomegalovirus in the Neonate: Immune Correlates of Infection and Protection

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