VIOLOGEN DYE INHIBITION OF METHANE FORMATION BY METHANOBACILLUS OMELIANSKII

Author:

Wolin E. A.1,Wolfe R. S.1,Wolin M. J.1

Affiliation:

1. Departments of Dairy Science and Microbiology, University of Illinois, Urbana, Illinois

Abstract

Wolin , E. A. (University of Illinois, Urbana), R. S. Wolfe, and M. J. Wolin . Viologen dye inhibition of methane formation by Methanobacillus omelianskii . J. Bacteriol. 87: 993–998. 1964.—Low concentrations of methyl or benzyl viologen inhibit the formation of CH 4 from ethanol and CO 2 by washed cells of Methanobacillus omelianskii . Hydrogen, which is normally formed from ethanol, accumulates in greater quantities when CH 4 formation is inhibited by viologens. The viologens do not stimulate H 2 formation from ethanol in the absence of CO 2 . Inhibition of CH 4 formation by the viologens is not reversed by H 2 . A variety of other dyes and possible electron acceptors were tested for inhibition, and none was inhibitory in the same low-concentration range at which the viologens were effective.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference14 articles.

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3. Studies on the methane fermentation. VI. The influence of carbon dioxide reduction by molecular hydrogen. Proe;BARKER H. A.;Natl. Acad. Sci. U.,1943

4. BREED R. S. E. G. D. MURRAY AND N. R. SMITH. 1957. Bergey's manual of determinative bacteriology 7th ed. The Williams & Wilkins Co. Baltimore.

5. CLARK W. M. 1960. Oxidation-reduction potentials of organic systems. The Williams & Wilkins Co. Baltimore.

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