SNaPBceBcon : a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans-Reference-Cited by-同舟云学术

SNaPBceBcon : a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans

Published:2016-02 Issue:2 Volume:54 Page:483-488
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Araujo Ricardo¹²,Caramalho Rita¹²³,Coutinho Carla⁴,Sá-Correia Isabel⁴

Affiliation:

1. IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal

2. Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal

3. Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria

4. iBB−Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal

Abstract

ABSTRACT We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans . Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.

Funder

Portuguese Foundation for Science and Technology

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02476-15

Reference31 articles.

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2. Microbiology of airway disease in patients with cystic fibrosis

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4. Evolving epidemiology of Pseudomonas aeruginosa and the Burkholderia cepacia complex in cystic fibrosis lung infection

5. Variation of the antimicrobial susceptibility profiles of Burkholderia cepacia complex clonal isolates obtained from chronically infected cystic fibrosis patients: a five-year survey in the major Portuguese treatment center

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