Author:
De Vos G F,Finan T M,Signer E R,Walker G C
Abstract
Transposon Tn5 encodes streptomycin resistance in addition to kanamycin-neomycin resistance. This resistance was not detectable in Escherichia coli but was efficiently expressed in Rhizobium meliloti and certain other strains. By analysis of cloned Tn5 restriction endonuclease fragments, the streptomycin resistance (str) gene was located in the right-hand side of the central region as the transposon is conventionally drawn. Transcription of str appeared to originate at pL, the promoter for the neo gene (neomycin phosphotransferase type II). Expression of streptomycin resistance in E. coli was obtained after cloning of the neo-str region downstream of a strong E. coli promoter. A construct in which PL was deleted also showed differential expression of streptomycin resistance.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
1. Structural analysis of Tn 5;Auerswald E.;Cold Spring Harbor Symp. Quant. Biol.,1980
2. Nucleotide sequence and exact location of the neomycin phosphotransferase gene from transposon Tn 5;Beck E.;Gene,1982
3. The procaryotic transposon TnS;Berg D. E.;Bio/Technology,1983
4. Transposition of R factor genes to bacteriophage lambda;Berg D. E.;Proc. Natl. Acad. Sci. U.S.A.,1975
5. Insertion, excision, and inversion of TnS;Berg D. E.;Cold Spring Harbor Symp. Quant. Biol.,1980
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