Comparison of Direct Genome Restriction Enzyme Analysis and Pulsed-Field Gel Electrophoresis for Typing of Vibrio vulnificus and Their Correspondence with Multilocus Sequence Typing Data-Reference-Cited by-同舟云学术

Comparison of Direct Genome Restriction Enzyme Analysis and Pulsed-Field Gel Electrophoresis for Typing of Vibrio vulnificus and Their Correspondence with Multilocus Sequence Typing Data

Published:2007-11-15 Issue:22 Volume:73 Page:7494-7500
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

González-Escalona Narjol¹²,Whitney Brooke¹,Jaykus Lee-Ann¹,DePaola Angelo²

Affiliation:

1. Department of Food Science, North Carolina State University, Raleigh, North Carolina

2. FDA Gulf Coast Seafood Laboratory, Dauphin Island, Alabama

Abstract

ABSTRACT We compared the potential of direct genome restriction enzyme analysis (DGREA) and pulsed-field gel electrophoresis (PFGE) for discriminating Vibrio vulnificus isolates from clinical (23) and environmental (17) sources. The genotypes generated by both methodologies were compared to previous multilocus sequence typing (MLST) data. DGREA established clearer relationships among V. vulnificus strains and was more consistent with MLST than with PFGE. DGREA is a very promising tool for epidemiological and ecological studies of V. vulnificus .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00738-07

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