Detection of Mycobacterium avium subsp. paratuberculosis in Drinking Water and Biofilms by Quantitative PCR

Author:

Beumer Amy1,King Dawn2,Donohue Maura2,Mistry Jatin3,Covert Terry4,Pfaller Stacy2

Affiliation:

1. University of Cincinnati, Raymond Walters College, Blue Ash, Ohio 45236

2. National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268

3. U.S. Environmental Protection Agency, Region 6, Dallas, Texas 75202

4. Shaw Environmental & Infrastructure Group, Cincinnati, Ohio 45268

Abstract

ABSTRACT It has been suggested that Mycobacterium avium subspecies paratuberculosis has a role in Crohn's disease. The organism may be acquired but is difficult to culture from the environment. We describe a quantitative PCR (qPCR) method to detect M. avium subsp. paratuberculosis in drinking water and the results of its application to drinking water and faucet biofilm samples collected in the United States.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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