Cloning and Sequence Analysis of Genes Encoding Staphylococcus hyicu s Exfoliative Toxin Types A, B, C, and D

Abstract

ABSTRACT Exfoliative toxins produced by certain strains of Staphylococcus hyicus mediate exudative epidermitis in pigs. In this study the genes coding for four different exfoliative toxin from S. hyicus (ExhA, ExhB, ExhC, and ExhD) were cloned and sequenced. The coding sequence of the four toxin genes ranged from 816 to 834 bp. The amino acid sequences of these four toxins were homologous to the earlier described exfoliative toxins SHETB from S. hyicus and ETA, ETB, and ETD from Staphylococcus aureus . The homology between the S. hyicus toxins was at the same level as the homology to the exfoliative toxins from S. aureus . The toxins showed similarity to serine proteases, including preservation of the catalytic tract in ExhA, ExhB, and ExhC. However, in ExhD, Asp in the putative catalytic tract was replaced with Glu. The recombinant toxins could be expressed in Escherichia coli , and three of the four toxins were recognized by monoclonal antibodies raised against native exfoliative toxins.