Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR-Reference-Cited by-同舟云学术

Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR

Published:1996-04 Issue:4 Volume:34 Page:980-982
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cogswell F B¹,Bantar C E¹,Hughes T G¹,Gu Y¹,Philipp M T¹

Affiliation:

1. Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA.

Abstract

It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The inhibitor is the host DNA itself. An acceptable value for analytical sensitivity can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.4.980-982.1996

Reference14 articles.

1. Assaying proteinases with Azocoll;Chivara R.;Anal. Biochem.,1984

2. Bloodstream invasion in early Lyme disease: results from a prospective, controlled, blinded study using the polymerase chain reaction;Goodman J. L.;Am. J. Med.,1995

3. The relapsing fever agent Borrelia hermsii has multiple copies of its chromosome and linear plasmids;Kitten T.;Genetics,1992

4. Isolation of Borrelia burgdorferi from biopsy specimens taken from healthy-looking skin of patients with Lyme borreliosis;Kuiper H.;J. Clin. Microbiol.,1994

5. Detection of Borrelia burgdorferi sensu lato in lesional skin of patients with erythema migrans and acrodermatitis chronica atrophicans by ospAspecific PCR;Moter S. E.;J. Clin. Microbiol.,1994

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