Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA-Reference-Cited by-同舟云学术

Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA

Published:1996-04 Issue:4 Volume:34 Page:986-987
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

da Silva A J¹,Schwartz D A¹,Visvesvara G S¹,de Moura H¹,Slemenda S B¹,Pieniazek N J¹

Affiliation:

1. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.

Abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.4.986-987.1996

Reference10 articles.

1. Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber's modified trichrome and Uvitex 2B stains;DeGirolami P. C.;J. Clin. Microbiol.,1995

2. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases;Fedorko D. P.;J. Clin. Microbiol.,1995

3. Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR;Franzen C.;J. Clin. Microbiol.,1995

4. Genetic evidence for the occurrence of extra-intestinal Enterocytozoon bieneusi infections;Hartskeerl R. A.;Nucleic Acids Res.,1993

5. Microsporidiosis in HIV positive patients: current methods for diagnosis using biopsy, cytologic, ultrastructural, immunological and tissue culture techniques;Schwartz D. A.;Folia Parasitol.,1994

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