Rapid antigen detection assay for identification of Burkholderia (Pseudomonas) pseudomallei infection

Author:

Anuntagool A1,Intachote P1,Naigowit P1,Sirisinha S1

Affiliation:

1. Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand.

Abstract

A simple antigen detection test was developed for rapid diagnosis of melioidosis caused by Burkholderia (Pseudomonas) pseudomallei infection. The method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for B. pseudomallei. The results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

1. Anuntagool N. P. Intachote T. Tharakul W. Chaowagul and S. Sirisinha. 1995. Re-evaluation of the significance of specific IgM and IgG antibody response in clinical melioidosis abstr. 208 p. 227. In Abstract book of the 2nd Asian Pacific Congress on Allergology and Clinical Immunology 1995. Taipei Taiwan.

2. Identification of specific antigens of Pseudomonas pseudomallei and evaluation of their efficacies for diagnosis of melioidosis;Anuntagool N.;J. Clin. Microbiol.,1993

3. Melioidosis: the tip of the iceberg;Dance D. A.;Clin. Microbiol. Rev.,1991

4. Detection of Pseudomonas pseudomallei antigen in urine for the diagnosis of melioidosis;Desakorn V.;Am. J. Trop. Med. Hyg.,1994

5. Monoclonal antibodies to Pseudomonas pseudomallei and their potential for diagnosis of melioidosis;Rugdech P.;Am. J. Trop. Med. Hyg.,1995

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