Effect of macrophage colony-stimulating factor on anticryptococcal activity of bronchoalveolar macrophages: synergy with fluconazole for killing

Author:

Brummer E1,Nassar F1,Stevens D A1

Affiliation:

1. Department of Medicine, Santa Clara Valley Medical Center, San Jose, California 95128-2699.

Abstract

The anticryptococcal activity of murine bronchoalveolar macrophages (BAM) and their synergy with fluconazole (FCZ) was studied. BAM cultured with tissue culture medium for 48 to 72 h were fungicidal (24 to 39%) in a 3-h killing assay. However, net killing of Cryptococcus neoformans did not continue when culture time was extended to 24 h, although BAM were fungistatic (88 to 98%). Treatment with macrophage colony-stimulating factor (M-CSF; 5,000 U/ml, 48 h) did not significantly increase BAM killing of a low challenge dose in 3-h assays compared with control BAM. However, M-CSF-treated BAM were significantly more fungistatic against higher challenge doses in the 3-h assays. FCZ was not fungicidal at 5 micrograms/ml but was highly fungistatic (98 and 99% at 24 and 48 h, respectively). M-CSF-treated BAM acted synergistically with FCZ (2.5 micrograms/ml) for significantly greater killing than control BAM, 55% versus 20% and 96% versus 45% at 24 h and 48 h, respectively. Killing by M-CSF BAM and FCZ (5.0 micrograms/ml) was significantly (P < 0.01) greater than that by control BAM and FCZ at 48 h. These findings indicate an important collaborative role for BAM and FCZ in killing C. neoformans, and this is enhanced by M-CSF.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference24 articles.

1. Effects of granulocyte macrophage-colony stimulating factor and colonystimulating factor 1 on the proliferation and differentiation of murine alveolar macrophages;Akagawa K. S.;J. Immunol.,1988

2. Regulation of colony-stimulating activity production: interactions of fibroblasts, mononuclear phagocytes, and lactoferrin;Bagby G. C.;J. Clin. Invest.,1983

3. Macrophages specifically regulate the concentration of their own growth factor in circulation;Bartocci A.;Proc. Natl. Acad. Sci. USA,1987

4. Activation of pulmonary macrophages for fungicidal activity by gamma-interferon or;Brummer E.;Iymphokines. Clin. Exp. Immunol.,1987

5. Candidacidal mechanisms of peritoneal macrophages activated with Iymphokines or gammainterferon;Brummer E.;J. Med. Microbiol.,1989

Cited by 21 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Specific Immunity and Cytokines;Cryptococcus neoformans;2014-04-30

2. Antifungal Agents and Antifungal Susceptibility Testing;Topley & Wilson's Microbiology and Microbial Infections;2010-03-15

3. Drug Resistance in Cryptococcus neoformans;Antimicrobial Drug Resistance;2009

4. Interferon‐γ as an Antifungal;The Journal of Infectious Diseases;2006-09-15

5. Efficacy of Recombinant Gamma Interferon for Treatment of Systemic Cryptococcosis in SCID Mice;Antimicrobial Agents and Chemotherapy;2001-03

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3