Affiliation:
1. Department of Immunology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
Abstract
ABSTRACT
The nucleotide sequences of a segment of the
Pneumocystis
mitochondrial large-subunit (mt LSU) rRNA gene from rhesus macaques coinfected with simian immunodeficiency virus (SIV) and
Pneumocystis carinii
were examined. Of 12 isolates examined, 3 were found to be identical and the others showed substantial sequence variation, with up to 13% divergence among variants. We identified two general sequence types that differed at several sites, including a conserved 26-nucleotide insertion. Four monkeys had evidence of two
Pneumocystis
variants present simultaneously, indicative of a mixed infection. There was a high degree of variance between the rhesus macaque-derived
Pneumocystis
mt LSU rRNA gene sequence and the cognate sequences in
Pneumocystis
organisms derived from other hosts. Analysis of the mt LSU rRNA genes of
Pneumocystis
organisms derived from rhesus macaques and several other mammalian hosts supports the observation that rhesus macaque-derived
Pneumocystis
is most closely related to human-derived
Pneumocystis
. In addition, the data identify the mt LSU rRNA gene as an informative locus for transmission and epidemiological studies of the SIV-rhesus macaque model of
Pneumocystis
infection.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Cited by
10 articles.
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