Standardized One-Step Real-Time Reverse Transcription-PCR Assay for Universal Detection and Quantification of Hepatitis Delta Virus from Clinical Samples in the Presence of a Heterologous Internal-Control RNA-Reference-Cited by-同舟云学术

Standardized One-Step Real-Time Reverse Transcription-PCR Assay for Universal Detection and Quantification of Hepatitis Delta Virus from Clinical Samples in the Presence of a Heterologous Internal-Control RNA

Published:2012-06 Issue:6 Volume:50 Page:2126-2128
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Scholtes Caroline¹²³,Icard Vinca¹,Amiri Majid³⁴,Chevallier-Queyron Philippe¹³,Trabaud Mary-Anne¹,Ramière Christophe²³,Zoulim Fabien³⁴⁵,André Patrice¹²³,Dény Paul⁶⁵

Affiliation:

1. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratoire de Virologie, Lyon, France

2. INSERM, U851, Lyon, France

3. Université de Lyon, Université Lyon 1, Lyon, France

4. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Service d'Hépato-Gastroentérologie, Lyon, France

5. Centre de Recherches en Cancérologie de Lyon, Department Immunity, Microenvironment Virus, UMR INSERM, U1052 UMR CNRS 5286, Lyon, France

6. Université Paris 13, Assistance Publique—Hôpitaux de Paris, Service de Bactériologie, Virologie-Hygiène, Hôpital Avicenne, Laboratoire associé au Centre National de Référence des Hépatites B, C et delta, Bobigny, France

Abstract

ABSTRACT As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro -transcribed RNAs as standards. Its linearity range is 500 to 1.7 × 10 11 copies/ml, its sensitivity is around 150 copies/ml, its repeatability is around 15%, and its reproducibility is below 0.25 log 10 copies/ml.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.06829-11

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