Author:
Soong M M,Iijima S,Wang L H
Abstract
The mechanism of cellular src (c-src) transduction by a transformation-defective deletion mutant, td109, of Rous sarcoma virus was studied by sequence analysis of the recombinational junctions in three td109-derived recovered sarcoma viruses (rASVs). Our results show that two rASVs have been generated by recombination between td109 and c-src at the region between exons 1 and 2 defined previously. Significant homology between td109 and c-src sequences was present at the sites of recombination. The viral and c-src sequence junction of the third rASV was formed by splicing a cryptic donor site at the 5' region of env of td109 to exon 1 of c-src. Various lengths of c-src internal intron 1 sequences were incorporated into all three rASV genomes, which resulted from activation of potential splice donor and acceptor sites. The incorporated intron 1 sequences were absent in the c-src mRNA, excluding its being the precursor for recombination with td109 and implying that initial recombinations most likely took place at the DNA level. A potential splice acceptor site within the incorporated intron 1 sequences in two rASVs was activated and was used for the src mRNA synthesis in infected cells. The normal env mRNA splice acceptor site was used for src mRNA synthesis for the third rASV.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference57 articles.
1. Bishop J. M. and H. Varmus. 1982. Functions and origins of retroviral transforming genes. p. 999-1108. In R. Weiss N. Teich H. Varmus and J. Coffin (ed.) RNA tumor viruses. Molecular biology of tumor viruses 2nd ed. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
2. Intricate combinational patterns of exon splicing generate multiple regulated troponin T isoforms from a single gene;Breitbart R. E.;Cell,1985
3. Rous sarcoma virus encodes a transcriptional activator;Broome S.;Cell,1985
4. Cloning and nucleotide sequences of cDNAs spanning the splice junctions of Rous sarcoma virus mRNAs;Chang L.;J. Virol.,1985
5. Neural tissues express high levels of the cellular src gene product pp 60csrc;Cotton P. C.;Mol. Cell. Biol.,1983
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献