ASSAY OF POLY-β-HYDROXYBUTYRIC ACID-Reference-Cited by-同舟云学术

ASSAY OF POLY-β-HYDROXYBUTYRIC ACID

Published:1961-07 Issue:1 Volume:82 Page:33-36
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Law John H.¹,Slepecky Ralph A.²

Affiliation:

1. Department of Chemistry, Harvard University, Cambridge, Massachusetts

2. Department of Biological Sciences, Northwestern University, Evanston, Illinois

Abstract

Law, John H. (Harvard University, Cambridge, Mass.) and Ralph A. Splepecky . Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82: 33–36. 1961—A convenient spectrophotometric assay of bacterial poly-β-hydroxybutyric acid has been devised. Quantitative conversion of poly-β-hydroxybutyric acid to crotonic acid by heating in concentrated sulfuric acid and determination of the ultraviolet absorption of the produce permits an accurate determination of this material in quantities down to 5 μg. This method has been used to follow the production of poly-β-hydroxybutyric acid by Bacillus megaterium strain KM.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.82.1.33-36.1961

Reference15 articles.

1. Role of poly-i-hydroxybutyric acid in the assimilation of organic carbon by bacteria;DOUDOROFF M.;Nature,1959

2. Occurrence of poly-6-hydroxybutyric acid in aerobic gram-negative bacteria;FORSYTH W. G.;Nature,1958

3. Poly-,3-hydroxybutyrate inclusions in the classification of aerobic gramnegative bacteria;HAWARD A. C.;J. Gen. Microbiol. 21:ii-iii.,1959

4. Synthesis and breakdown of poly-iS-hydroxybutyric acid by bacteria;HAWARD A. C.;J. Gen. Microbiol.,1959

5. Sudanophilic granules and lipid of Pseudomonas methanica;KALLIO R. E.;J. Bacteriol.,1960

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