Affiliation:
1. Department of Microbiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
Abstract
Rickettsia quintana
grew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H
2
O
2
, viz., catalase, potassium pyruvate, or charcoal, or by the reducing compounds sodium sulfite and sodium thioglycollate.
R. quintana
was catalase-negative, but no H
2
O
2
production could be demonstrated by the catalase-aminotriazole technique. A minimum inoculum giving 10
5
cells/ml was required to initiate growth. The generation time at 33 C was 10 hr. The temperature range for growth was 28 to 37 C. Growth was enhanced when succinate or glutamate was added as energy source.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference19 articles.
1. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase;Beers R. F.;J. Biol. Chem.,1952
2. Study on the growth of rickettsiae. I. A tissue culture system for quantitative estimations of R. tsutsugamushi;Bozeman F. M.;J. Immunol.,1956
3. Catalase-aminotriazole method for measuring secretion of hydrogen peroxide by microorganisms;Cohen G.;J. Bacteriol.,1969
4. Studies on isoniazid and tubercle bacilli. II. The growth requirements, catalase activities, and pathogenic properties of isoniazid-resistant mutants;Cohn M. L.;Amer. Rev. Tuberc.,1954
5. The effects of some sulfhydryl compounds on growth of catalasepositive and catalase-negative tubercle bacilli;Coleman C. M.;Amer. Rev. Tuberc.,1955
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