Mutants of Escherichia coli K-12 Blocked in the Final Reaction of Ubiquinone Biosynthesis: Characterization and Genetic Analysis

Author:

Stroobant P.1,Young I. G.1,Gibson F.1

Affiliation:

1. Department of Biochemistry, John Curtin School of Medical Research, The Institute of Advanced Studies, Australian National University, Canberra, A.C.T., Australia

Abstract

The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference14 articles.

1. Aplin R. T. and W. T. Pike. 1966. Mass spectra of 1 4- dihydroxybenzenes and p-benzoquinones: quinol-quinone interconversion in the heated inlet system of a mass spectrometer. Chem. Ind. (London) p. 2009.

2. Mutant strains of Escherichia coli K-12 unable to form ubiquinone;Cox G. B.;J. Bacteriol.,1968

3. Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3- octapreny1-4-hydroxybenzoic acid and 2-octaprenylphenol;Cox G. B.;J. Bacteriol.,1969

4. Genetic control of the L-a-glycerophosphate system in Escherichia coli;Cozzarelli N. R.;J. Mol. Biol.,1968

5. New multiprenylquinones in the biosynthesis of ubiquinone;Friis P.;Biochem. Biophys. Res. Commun.,1967

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