Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens-Reference-Cited by-同舟云学术

Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens

Published:2009-04 Issue:4 Volume:47 Page:1206-1208
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hendry Cindy¹,Dionne Kim¹,Hedgepeth Annie¹,Carroll Karen¹,Parrish Nicole¹

Affiliation:

1. The Johns Hopkins Medical Institutions, Baltimore, Maryland

Abstract

ABSTRACT Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time (∼2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02097-08

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