Involvement of the cyclic AMP-responsive element binding protein in bovine leukemia virus expression in vivo

Author:

Adam E1,Kerkhofs P1,Mammerickx M1,Kettmann R1,Burny A1,Droogmans L1,Willems L1

Affiliation:

1. Department of Molecular Biology, University of Brussels, Rhode-St-Genèse, Belgium.

Abstract

The TAR element (Tax-responsive element; also called TxRE) is a major determinant of the regulation of bovine leukemia virus (BLV) expression. In order to gain insight into the mechanisms of viral expression, complexes formed between proteins and the TAR enhancer DNA were analyzed by gel retardation assays. We report here that nuclear lysates from ex vivo-isolated B lymphocytes contain proteins that specifically bind to TAR. An antibody directed toward the cyclic AMP-responsive element binding (CREB) protein supershifted a complex (C1) present only in BLV-infected B lymphocytes. The CREB protein thus appears to be a major transcription factor involved in BLV expression in vivo.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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