Immunization with Potato Plants Expressing VP60 Protein Protects against Rabbit Hemorrhagic Disease Virus-Reference-Cited by-同舟云学术

Immunization with Potato Plants Expressing VP60 Protein Protects against Rabbit Hemorrhagic Disease Virus

Published:1999-05 Issue:5 Volume:73 Page:4452-4455
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Castañón S.¹,Marín M. S.²,Martín-Alonso J. M.²,Boga J. A.²,Casais R.²,Humara J. M.¹,Ordás R. J.¹,Parra F.²

Affiliation:

1. Departamento de Biologı́a de Organismos y Sistemas1 and

2. Departamento de Bioquı́mica y Biologı́a Molecular,2 Instituto Universitario de Biotecnologı́a de Asturias (CSIC), Universidad de Oviedo, 33006 Oviedo, Spain

Abstract

ABSTRACT The major structural protein VP60 of rabbit hemorrhagic disease virus (RHDV) has been produced in transgenic potato plants under the control of a cauliflower mosaic virus 35S promoter or a modified 35S promoter that included two copies of a strong transcriptional enhancer. Both types of promoters allowed the production of specific mRNAs and detectable levels of recombinant VP60, which were higher for the constructs carrying the modified 35S promoter. Rabbits immunized with leaf extracts from plants carrying this modified 35S promoter showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.73.5.4452-4455.1999

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