Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA

Author:

Velázquez Encarna1,Martı́nez-Romero Esperanza2,Rodrı́guez-Navarro Dulce Nombre3,Trujillo Martha E.1,Daza Antonio3,Mateos Pedro F.1,Martı́nez-Molina Eustoquio1,van Berkum Peter4

Affiliation:

1. Departamento de Microbiologı́a y Genética, Universidad de Salamanca, Salamanca,1 and

2. Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México2; and

3. Centro de Investigación y Desarollo Agrario. CIDA “Las Torres y Tomejil,” Seville,3 Spain;

4. U.S. Department of Agriculture, Agricultural Research Service, Soybean and Alfalfa Research Laboratory, Beltsville, Maryland 207054

Abstract

ABSTRACT Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli , Rhizobium gallicum , Rhizobium giardinii , Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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