Affiliation:
1. Departments of Medicine
2. Departments of Microbiology and Immunology
3. Medicine
4. Pathology and Laboratory Medicine, Indiana University, Indianapolis, Indiana 46202
5. Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Abstract
ABSTRACT
The ability to bind extracellular matrix proteins is a critical virulence determinant for skin pathogens.
Haemophilus ducreyi
, the etiological agent of the genital ulcer disease chancroid, binds extracellular matrix components, including fibronectin (FN). We investigated
H. ducreyi
FN binding and report several important findings about this interaction. First, FN binding by
H. ducreyi
was greatly increased in bacteria grown on heme and almost completely inhibited by hemoglobin. Second, wild-type strain 35000HP bound significantly more FN than did a
dsrA
mutant in two different FN binding assays. Third, the expression of
dsrA
in the
dsrA
mutant restored FN binding and conferred the ability to bind FN to a non-FN-binding
Haemophilus influenzae
strain. Fourth, an anti-DsrA monoclonal antibody partially blocked FN binding by
H. ducreyi
. The hemoglobin receptor, the collagen-binding protein, the
H. ducreyi
lectin, the fine-tangle pili, and the outer membrane protein OmpA2 were not involved in
H. ducreyi
FN binding, since single mutants bound FN as well as the parent strain did. However, the major outer membrane protein may have a minor role in FN binding by
H. ducreyi
, since a double
dsrA momp
mutant bound less FN than did the single
dsrA
mutant. Finally, despite major sequence differences, DsrA proteins from both class I and class II
H. ducreyi
strains mediated FN and vitronectin binding. We concluded that DsrA is the major factor involved in FN binding by both classes of
H. ducreyi
strains.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献