Comparison of Genotyping Helicobacter pylori Directly from Biopsy Specimens and Genotyping from Bacterial Cultures

Author:

Park Chang-Young12,Kwak Minjung3,Gutierrez Oscar4,Graham David Y.1,Yamaoka Yoshio1

Affiliation:

1. Department of Medicine, Veterans Affairs Medical Center, and Baylor College of Medicine, Houston, Texas

2. Department of Internal Medicine, Kangbuk Samsung Hospital, Sunkyunkwan University School of Medicine, Seoul

3. Department of Information of Statistics, Pyongtaek University, Pyongtaek, Korea

4. Department of Medicine, Universidad Nacional de Colombia, Bogota, Colombia

Abstract

ABSTRACT PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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