Affiliation:
1. Division of Infectious Diseases, Department of Medicine
2. Infectious Diseases Research Laboratory
3. Division of Biostatistics
4. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota
Abstract
ABSTRACT
The activity of electrical current against planktonic bacteria has previously been demonstrated. The short-term exposure of the bacteria in biofilms to electrical current in the absence of antimicrobials has been shown to have no substantial effect; however, longer-term exposure has not been studied. A previously described in vitro model was used to determine the effect of prolonged exposure (i.e., up to 7 days) to low-intensity (i.e., 20-, 200-, and 2,000-microampere) electrical direct currents on
Pseudomonas aeruginosa
,
Staphylococcus aureus
, and
Staphylococcus epidermidis
biofilms. Dose- and time-dependent killing was observed. A maximum of a 6-log
10
-CFU/cm
2
reduction was observed when
S. epidermidis
biofilms were exposed to 2,000 microamperes for at least 2 days. A 4- to 5-log
10
-CFU/cm
2
reduction was observed when
S. aureus
biofilms were exposed to 2,000 microamperes for at least 2 days. Finally, a 3.5- to 5-log
10
-CFU/cm
2
reduction was observed when
P. aeruginosa
biofilms were exposed to electrical current for 7 days. A higher electrical current intensity correlated with greater decreases in viable bacteria at all time points studied. In conclusion, low-intensity electrical current substantially reduced the numbers of viable bacteria in staphylococcal or
Pseudomonas
biofilms, a phenomenon we have labeled the “electricidal effect.”
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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