Encephalomyocarditis Virus Infection of Mouse Plasmacytoma Cells I. Inhibition of Cellular Protein Synthesis

Author:

Lawrence Charles1,Thach Robert E.1

Affiliation:

1. Department of Biological Chemistry, Division of Biology and Biomedical Sciences, Washington University, Saint Louis, Missouri 63110

Abstract

Mouse plasmacytoma ascites tumor cells (MOPC 460) were efficiently infected with encephalomyocarditis virus. Inhibition of host protein synthesis was evident after 2 h and complete by 4 h postinfection. The mechanism by which virus infection results in inhibition of host cell protein synthesis was studied in vitro. Cell-free protein-synthesizing systems, prepared from uninfected and infected cells, were found to be equally active with respect to their abilities to translate cellular and viral mRNAs. The plasmacytoma cell-free system was also shown to be insensitive to the addition of double-stranded viral RNA. Host cellular mRNA was isolated from uninfected and infected cells. No difference in the amount or size distribution of the mRNA was detected. However, the mRNA from infected cells was translated only 46 to 49% as actively as that from uninfected cells. mRNA isolated from cells in which initiation of protein synthesis was inhibited with pactamycin was similarly inactivated. Simultaneous addition of viral RNA and cellular mRNA to the plasmacytoma cell-free system resulted in a complete suppression of the translation of the cellular message, whereas viral RNA was translated normally.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference42 articles.

1. Studies on the mechanism of poliovirus-induced cell damage I. The relation between poliovirus-induced metabolic and morphological alterations in cultured cells;Bablanian R.;Virology,1965

2. Baltimore D. 1969. The replication of picornaviruses p. 101-176. In H. B. Levy (ed.) The biochemistry of viruses. Marcel Dekker Inc. New York.

3. Early functions of the genome of herpes virus. II. Inhibition of the formation of cell-specific polysomes;Ben-Porat T.;Virology,1971

4. Boime I. and H. Aviv. 1973. Cell-free synthesis of viral proteins in the Krebs II ascites tumor system p. 187-215. In A. Laskin and J. Last (ed.) Methods in molecular biology vol. 4. Marcel Dekker Inc. New York.

5. Protein synthesis directed by encephalomyocarditis virus RNA II. The in vitro synthesis of high molecular weight proteins and elements of the viral capsid;Boime I.;Biochem. Biophvs. Res. Commun.,1971

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