Isolation and Partial Characterization of Nucleic Acid of Influenza Virus

Author:

Nayak D. P.1,Baluda M. A.1

Affiliation:

1. Department of Medical Microbiology and Immunology, School of Medicine, University of California, Los Angeles, California 90024

Abstract

The principal ribonucleic acid (RNA) component isolated from purified equine influenza virus has an approximate sedimentation coefficient ( S 20,W ) of 21 S in sucrose gradient containing 0.1 m NaCl. Three other components of 18 S , 14 S , and 8 S were also detected. All the RNA components have characteristics of single-stranded RNA. The average base composition of the principal RNA components is cytosine, 22.2; adenine, 22.9; guanine, 22.3; and uridine, 32.6. There was no qualitative difference in the RNA isolated from noninfectious virus particles compared to that from infectious virions.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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