Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes

Author:

Qiu Dongru1,Fujita Kyoko2,Sakuma Yuko2,Tanaka Teruo3,Ohashi Yoshiaki1,Ohshima Hideyuki1,Tomita Masaru14,Itaya Mitsuhiro12

Affiliation:

1. Institute for Advanced Biosciences and Bioinformatics Program, Graduate School of Governance and Media, Keio University, Tsuruoka, Yamagata

2. Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo

3. Department of Marine Science and Technology, Tokai University, Shizuoka

4. Department of Environmental Information, Keio University, Fujisawa, Kanagawa, Japan

Abstract

ABSTRACT The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons ( rrn ) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPβ, skin element, and PBSX, as well as the insertion element IS 4Bsu 1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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