Rapid Differentiation of Mycobacterium tuberculosis and M. bovis by High-Resolution Melt Curve Analysis-Reference-Cited by-同舟云学术

Rapid Differentiation of Mycobacterium tuberculosis and M. bovis by High-Resolution Melt Curve Analysis

Published:2010-11 Issue:11 Volume:48 Page:4269-4272
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ereqat Suheir¹²,Bar-Gal Gila Kahila²,Nasereddin Abedelmajeed¹,Azmi Kifaya¹,Qaddomi Sharif E.¹,Greenblatt Charles L.³,Spigelman Mark³⁴,Abdeen Ziad¹

Affiliation:

1. Al-Quds Nutrition and Health Research Institute, Faculty of Medicine, Al-Quds University, Abu-Deis, P.O. Box 201760, West Bank, Palestine

2. the Koret School of Veterinary Medicine, the Hebrew University of Jerusalem, Rehovot, Israel

3. Department of Microbiology and Molecular Genetics, the Hebrew University-Hadassah Medical School, Jerusalem, Israel

4. Department of Infection, University College London, London, United Kingdom

Abstract

ABSTRACT Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00943-10

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