Author:
Rönnblom L,Forsgren A,Alm G V
Abstract
All of 23 different preparations of formaldehyde-fixed and heat-killed bacteria induced the appearance of high levels of interferon (IFN) in cultures of human peripheral blood mononuclear leukocytes. Some bacteria induced peak IFN titers after 24 h of culture, whereas other bacteria showed maximal titers on culture days 2 to 3. The IFN displayed various properties. One type, which appeared early during the cultures, had characteristics of IFN-alpha, being resistant to pH 2 treatment but neutralized by antibodies to IFN-alpha. A second type, which appeared later, on culture days 2 to 3, resembled IFN-gamma in being sensitive to pH 2 treatment but resistant to anti-IFN-alpha antibodies. A third type, which appeared to be sensitive to both pH 2 and antibody treatment, was interpreted as atypical IFN-alpha. The application of cell fractionation procedures indicated that nonadherent, predominantly Fc receptor-bearing, non-T, non-B cells were producers of IFN-alpha as defined by its antigenic properties. They copurified approximately with cells carrying natural killer activity toward human erythroid leukemia K562 cells. Some bacteria apparently also stimulated T lymphocytes to produce material with properties of IFN-gamma.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference29 articles.
1. Many bacterial species are mitogenic for human blood B;Banck G.;Iymphocytes. Scand. J. Immunol.,1978
2. Interferon production in cocultures between mouse spleen cells and tumor cells. Possible role of mycoplasmas in interferon induction;Beck J.;J. Immunol. Methods,1980
3. Mycoplasma contamination in human tumor cell lines: effect on interferon induction and susceptibility to natural killing;Birke C.;J. Immunol.,1981
4. Isolation of mononuclear cells and granulocytes from human blood;Biyum A.;Scand. J. Clin. Lab. Invest.,1968
5. Interferons induced by Chlamydia trachomatis in human Iymphocyte cultures;Duc-Goiran P.;Biomedicine,1981
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献