Occurrence of 2-keto-deoxyoctonic acid 5-phosphate in lipopolysaccharides of Vibrio cholerae Ogawa and Inaba-Reference-Cited by-同舟云学术

Occurrence of 2-keto-deoxyoctonic acid 5-phosphate in lipopolysaccharides of Vibrio cholerae Ogawa and Inaba

Published:1985-02 Issue:2 Volume:161 Page:795-798
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Brade H

Abstract

A phosphorylated 2-keto-3-deoxyoctonic acid (KDO) was released from the lipopolysaccharides of Vibrio cholerae Ogawa and Inaba after strong acid hydrolysis. The phosphorylated KDO was identified by gas-liquid chromatography and mass spectrometry after reduction and permethylation as KDO-5-phosphate and an isomer of it being phosphorylated at position 7 or 8. After treatment with alkaline phosphatase, KDO was detected by gas-liquid chromatography and mass spectrometry. It was indistinguishable from authentic 2-keto-3-deoxy-D-manno-octonic acid.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.161.2.795-798.1985

Reference18 articles.

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2. A method to detect 2-keto-3- deoxy-octanat and related compounds on pherograms and chromatograms;Brade H.;Anal. Biochem.,1983

3. A new lipopolysaccharide antigen identified in Acinetobacter calcoaceticus: occurrence of widespread natural antibody;Brade H.;J. Med. Microbiol.,1983

4. Common lipopolysaccharide specificity: new type of antigen residing in the inner core region of S- and R-form lipopolysaccharides from different families of gram-negative bacteria;Brade H.;Infect. Immun.,1983

5. Differential determination of the 3-deoxy-D-mannooctulosonic acid residues in lipopolysaccharides of Salmonella minnesota rough mutants;Brade H.;Eur. J. Biochem.,1983

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