Characterization of a Culturable “ Gastrospirillum hominis ” ( Helicobacter heilmannii ) Strain Isolated from Human Gastric Mucosa

Author:

Andersen L. P.1,Boye K.2,Blom J.3,Holck S.4,Nørgaard A.15,Elsborg L.5

Affiliation:

1. Department of Clinical Microbiology, National University Hospital (Rigshospitalet),1 and

2. Department of Clinical Biochemistry2and

3. Department of Molecular Cell Biology,3 Statens Serum Institut, Copenhagen, and

4. Department of Pathology4and

5. Department of Medicine B,5Hillerød Sygehus, Hillerød, Denmark

Abstract

ABSTRACT Spiral organisms were isolated from an antral gastric mucosal biopsy specimen from a dyspeptic patient with gastritis. Only corkscrew-shaped organisms resembling “ Gastrospirillum hominis ” (“ Helicobacter heilmannii ”) but no Helicobacter pylori -like organisms were seen in histological sections. H. pylori was not cultured from specimens from this patient. On the basis of biochemical reactions, morphology, ultrastructure, and 16S DNA sequencing, the isolated “ G. hominis ” was shown to be a true Helicobacter sp. very similar to Helicobacter felis and the “ Gastrospirillum ” but was separate from H. pylori . “ G. hominis ” is a pleomorphic gram-negative cork-screw-shaped, motile rod with 3 to 8 coils and a wavelength of about 1 μm. In contrast to H. pylori , it has up to 14 sheathed flagellar uni- or bipolar fibrils but no periplasmic fibrils. “ G. hominis ” grows under microaerobic conditions at 36 and 41°C on 7% lysed, defibrinated horse blood agar plates within 3 to 7 days and can be subcultured under microaerobic but not under anaerobic conditions on media similar to those used for H. pylori and H. felis . The small translucent colonies were, in contrast to those of H. felis , indistinguishable from those of H. pylori . “ G. hominis ” is, like H. pylori and H. felis , motile, is oxidase, catalase, nitrite, nitrate, and urease positive, and produces alkaline phosphatase and arginine arylamidase. Like H. pylori and H. felis , it is sensitive to cephalothin (30-μg disc), resistant to nalidixic acid (30-μg disc), and sensitive to most other antibiotics. The 16S DNA sequence clusters “ G. hominis ” together with “ Gastrospirillum ,” H. felis , Helicobacter bizzozeronii , Helicobacter salmonii, Helicobacter nemestrinae , Helicobacter acinonychis , and H. pylori .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference44 articles.

1. Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique

2. Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis

3. Sulle ghiandole tubulari del tubo gastroenterico e sui rapporti del loro coll’epitelio de rivestimento della mucosa;Bizzozero G.;Atti R. Accad. Sci. Torino,1893

4. A study of Russell bodies in human monoclonal plasma cells by means of immunofluorescence and electron microscopy;Blom J.;Acta Pathol. Microbiol. Immunol. Scand. Sect. A,1971

5. Boye K. E. Høgdall and M. Borre. Identification of bacteria using two degenerate 16S rDNA sequencing primers. Microbiol. Res. in press.

Cited by 54 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3