Enhanced Display of Lipase on the Escherichia coli Cell Surface, Based on Transcriptome Analysis-Reference-Cited by-同舟云学术

Enhanced Display of Lipase on the Escherichia coli Cell Surface, Based on Transcriptome Analysis

Published:2010-02 Issue:3 Volume:76 Page:971-973
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Baek Jong Hwan¹²,Han Mee-Jung¹²³,Lee Seung Hwan¹,Lee Sang Yup¹²⁴

Affiliation:

1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center

2. Center for Systems and Synthetic Biotechnology and Institute for the BioCentury

3. Department of Chemical and Biomolecular Engineering, Dongyang University, 1 Kyochon-dong, Punggi-eup, Yeongju, Gyeongbuk 750-711, Republic of Korea

4. Department of Bio and Brain Engineering, Department of Biological Sciences and Bioinformatics Research Center, KAIST, Daejeon 305-701

Abstract

ABSTRACT A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02463-09

Reference23 articles.

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