Affiliation:
1. Department of Molecular Cell Biology, Utrecht University, The Netherlands.
Abstract
The structural gene encoding the 70-kDa outer membrane protein FrpB of Neisseria meningitidis was cloned and sequenced. A mutant lacking FrpB was constructed. No difference in iron utilization between the mutant and the parental strain was observed. A minor effect of the mutation on serum resistance was observed. A topology model for FrpB in the outer membrane is proposed.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference36 articles.
1. Iron acquisition by Neisseria meningitidis in vitro;Archibald F. S.;Infect. Immun.,1980
2. Ausubel F. M. R. Brent R. E. Kingston D. M. Moore J. G. Seidman J. A. Smith and K. Struhl. 1989. Current protocols in molecular biology. Greene Publishing Associates New York.
3. Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli;Bagg A.;Biochemistry,1987
4. Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae;Beucher M.;J. Bacteriol.,1995
5. Bitter W. H. Zomer P. J. Weisbeek and J. Tommassen. Unpublished data.
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