Affiliation:
1. Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
Abstract
The Bacillus subtilis acsA (acetyl coenzyme A synthetase) and acuABC (acetoin utilization) genes were previously identified in the region downstream from the ccpA gene, which encodes a protein required for catabolite repression of the amyE (alpha-amylase) gene. The acsA and acuABC genes are divergently transcribed, with only 20 bp separating the -35 sequences of their promoters. Expression of these genes was maximal in stationary phase and was repressed by the addition of glucose to the growth medium. Two sites resembling amyO, the cis-acting regulatory target site for amyE, were identified in the acsA and acuABC promoter regions. Glucose repression of acsA and acuABC transcription was dependent on both CcpA and the amyO-like sequences.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference32 articles.
1. The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli;Brown T. D. K.;J. Gen. Microbiol.,1977
2. Chambliss G. H. 1993. Catabolite repression p. 213-219. In A. L. Sonenshein J. A. Hoch and R. Losick (ed.) Bacillus subtilis and other gram-positive bacteria: biochemistry physiology and molecular genetics. American Society for Microbiology Washington D.C.
3. .Chambliss G. H. Personal communication.
4. Glutamine synthetase gene of Bacillus subtilis;Fisher S. H.;Gene,1984
5. Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein;Fujita Y.;J. Bacteriol.,1994
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