Mo-independent nitrogenase 3 is advantageous for diazotrophic growth of Azotobacter vinelandii on solid medium containing molybdenum-Reference-Cited by-同舟云学术

Mo-independent nitrogenase 3 is advantageous for diazotrophic growth of Azotobacter vinelandii on solid medium containing molybdenum

Published:1994-09 Issue:17 Volume:176 Page:5583-5586
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Maynard R H¹,Premakumar R¹,Bishop P E¹

Affiliation:

1. U.S. Department of Agriculture, Agriculture Research Service, Raleigh, North Carolina.

Abstract

Competition experiments between wild-type Azotobacter vinelandii and a mutant lacking Mo-independent nitrogenase 3 indicate that nitrogenase 3 provides an advantage during diazotrophic growth on agar media containing 100 to 500 nM Na2MoO4 but not in liquid media under the same conditions. Expression of nitrogenase 3 in wild-type cells growing on agar surfaces was verified with an anfH-lacZ fusion and by detection of nitrogenase 3 subunits. These results show that nitrogenase 3 is important for diazotrophic growth on agar medium at molybdenum concentrations that are not limiting for Mo-dependent diazotrophic growth in liquid medium.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.176.17.5583-5586.1994

Reference24 articles.

1. Bishop P. E. 1993. Three genetically distinct nitrogenase systems in Azotobacter vinelandii p. 301-324. In L. L. Barton and B. C. Hemming (ed.) Iron chelation in plants and soil microorganisms. Academic Press Inc. San Diego Calif.

2. Nitrogen fixation in molybdenum-deficient continuous culture by a strain of Azotobacter vinelandii carrying a deletion of the structural genes for nitrogenase (nifHDK);Bishop P. E.;Biochem. J.,1986

3. Evidence for an alternative nitrogen fixation system inAzotobacter vinelandii;Bishop P. E.;Proc. Natl. Acad. Sci. USA,1980

4. Genetics and molecular biology of alternative nitrogen fixation systems. Annu;Bishop P. E.;Rev. Plant Physiol. Plant Mol. Biol.,1990

5. Bishop P. E. and R. Premakumar. 1992. Alternative nitrogen fixation systems p. 736-762. In G. Stacy R. H. Burris and H. J.

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