Affiliation:
1. Laboratoire de Bactériologie, Université de Franche-Comté, Besançon, France.
Abstract
The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference39 articles.
1. Detection of resistance factors in fish pathogen Aeromonas liquefaciens;Aoki T.;J. Gen. Microbiol.,1971
2. Bernard P. P. Gabant M. Elbahassi and M. Couturier. Positive selection vectors using the ccdB killer gene of the factor F. Submitted for publication.
3. Conjugation system of Inc C plasmid RA1, and the interaction of RAl pili with specific RNA phage C-1;Bradley D. E.;Res. Microbiol.,1989
4. Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis;Casse F.;J. Gen. Microbiol.,1979
5. Mini-Pi plasmid replication: the autoregulation-sequestration paradox;Chattorai D. K.;Cell,1988
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