The presence of linoleic acid in Escherichia coli cannot be confirmed

Author:

Cronan J E1,Rock C O1

Affiliation:

1. Department of Microbiology, University of Illinois at Urbana-Champaign 61801.

Abstract

Escherichia coli was recently reported to accumulate significant quantities of linoleic acid in stationary phase (H. Rabinowitch, D. D. Sklan, D. H. Chace, R. D. Stevens, and I. Fridovich, J. Bacteriol. 175:5324-5328, 1993). Since this finding would have considerable impact on the biochemical mechanisms of type II fatty acid synthases, we have attempted to confirm this observation. We found no evidence for the accumulation of linoleic acid in late-stationary-phase cultures of E. coli and conclude that the results of Rabinowitch et al. are artifactual.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

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2. Christie W. W. 1982. Lipid analysis 2nd ed. Pergamon Press London.

3. Biotin deficiency and the fatty acids of certain biotin-requiring bacteria;Croom J. A.;J. Bacteriol.,1964

4. Davis R. W. D. Botstein and J. R. Roth. 1980. A manual for genetic engineering-advanced bacterial genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

5. Karkas J. H. Tuirer and E. Chargaff. 1964. Studies on the specification of accessory biochemical characters as exemplified by the fatty acid patterns of various strains of Escherichia coli. NOTES 3071

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