Author:
Kuechler Stefan Martin,Dettner Konrad,Kehl Siegfried
Abstract
ABSTRACTMany members of the suborder Heteroptera have symbiotic bacteria, which are usually found extracellularly in specific sacs or tubular outgrowths of the midgut or intracellularly in mycetomes. In this study, we describe the second molecular characterization of a symbiotic bacterium in a monophagous, seed-sucking stink bug of the family Lygaeidae (sensu stricto).Chilacis typhaepossesses at the end of the first section of the midgut a structure which is composed of circularly arranged, strongly enlarged midgut epithelial cells. It is filled with an intracellular endosymbiont. This “mycetocytic belt” might represent an evolutionarily intermediate stage of the usual symbiotic structures found in stink bugs. Phylogenetic analysis based on the 16S rRNA and thegroELgenes showed that the bacterium belongs to theGammaproteobacteria, and it revealed a phylogenetic relationship with a secondary bacterial endosymbiont ofCimex lectulariusand free-living plant pathogens such asPectobacteriumandDickeya. The distribution and ultrastructure of the rod-shapedChilacisendosymbiont were studied in adults and nymph stages using fluorescencein situhybridization (FISH) and electron microscopy. The detection of symbionts at the anterior poles of developing eggs indicates that endosymbionts are transmitted vertically. A new genus and species name, “CandidatusRohrkolberia cinguli,” is proposed for this newly characterized clade of symbiotic bacteria.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
48 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献