Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1 , Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288

Author:

Ito Takeshi1,Ikeya Tomonori1,Sahara Ken1,Bando Hisanori1,Asano Shin-ichiro1

Affiliation:

1. Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan

Abstract

ABSTRACT Two novel crystal protein genes, cry30Ba and cry44Aa , were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC 50 ] = 6 ng/ml) and Aedes aegypti (LC 50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference15 articles.

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