Affiliation:
1. Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands
Abstract
ABSTRACT
Biofiltration of industrial carbon disulfide (CS
2
)-contaminated waste air streams results in the acidification of biofilters and therefore reduced performance, high water use, and increased costs. To address these issues, we isolated 16 extremely acidophilic CS
2
-converting
Acidithiobacillus thiooxidans
strains that tolerated up to 6% (vol/vol) sulfuric acid. The ecophysiological properties of five selected strains (2Bp, Sts 4-3, S1p, G8, and BBW1) were compared. These five strains had pH optima between 1 (2Bp) and 2 (S1p). Their affinities for CS
2
ranged between 80 (G8) and 130 (2Bp) μM. Strains S1p, G8, and BBW1 had more hydrophobic cell surfaces and produced less extracellular polymeric substance than did strains 2Bp and Sts 4-3. All five strains converted about 80% of the S added as CS
2
to S
0
when CS
2
was supplied in excess. The rate of S
0
consumption varied between 7 (Sts 4-3) and 63 (S1p) nmol O
2
min
−1
ml culture
−1
. Low S
0
consumption rates correlated partly with low levels of cell attachment to externally produced S
0
globules. During chemostat growth, the relative amount of CS
2
hydrolase in the cell increased with decreasing growth rates. This resulted in more S
0
accumulation during CS
2
overloads at low growth rates. Intermittent interruptions of the CS
2
supply affected all five strains. Strains S1p, G8, and BBW1 recovered from 24 h of starvation within 4 h, and strains 2Bp and Sts 4-3 recovered within 24 h after CS
2
was resupplied. We recommend the use of mixtures of
Acidithiobacillus
strains in industrial biofilters.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献